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KMID : 0363320050260020409
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Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 2 p.409 ~ p.419
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Protective Effects of Boyanghwanoh-tang on Zinc-mediated Cytotoxicity in H9c2 Cardiomyoblast Cells
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Lee Yun-Jae
Rhim Eun-Kyung
Jeong Hyun-Ae
Shin Sun-Ho
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Abstract
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The water extract of Boyanghwanoh-tang has been used for treatment of ischemic vascular disease in oriental medicine. However, little is known about the mechanism by which the water extract of Boyanghwanoh-tang rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of Boyanghwanoh-tang on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that, after treatment of H9c2 cells with zinc, there was a decrease in cell viability in a dose dependent manner, and there was a chromatin condensation. Zinc induced the change of cell morphology. In addition, zinc induced mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of Boyanghwanoh-tang consistently with increase of the peroxoredoxin 1, 2, 3, 5, and 6 expression. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells, and that protective effects of Boyanghwanoh-tang against oxidative injuries are achieved through regulation of peroxiredoin expression.
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KEYWORD
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Boyanghwanoh-tang, cardiomyoblast, zinc, cytotoxicity
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